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GUS reporter system : ウィキペディア英語版 | GUS reporter system
The GUS reporter system (''GUS'': β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique. ==Purpose== The purpose of this technique is to analyze the activity of a promoter (in terms of expression of a gene under that promoter) either in a quantitative way or through visualization of its activity in different tissues. The technique is based on β-glucuronidase, an enzyme from the bacterium ''Escherichia coli''; this enzyme, when incubated with some specific colorless or non-fluorescent substrates, can transform them into coloured or fluorescent products.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「GUS reporter system」の詳細全文を読む
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